Berry and phenology-related traits in grapevine Vitis viniferaL.: From Quantitative Trait Loci to underlying genesReportar como inadecuado

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BMC Plant Biology

, 8:38

First Online: 17 April 2008Received: 02 July 2007Accepted: 17 April 2008


BackgroundThe timing of grape ripening initiation, length of maturation period, berry size and seed content are target traits in viticulture. The availability of early and late ripening varieties is desirable for staggering harvest along growing season, expanding production towards periods when the fruit gets a higher value in the market and ensuring an optimal plant adaptation to climatic and geographic conditions. Berry size determines grape productivity; seedlessness is especially demanded in the table grape market and is negatively correlated to fruit size. These traits result from complex developmental processes modified by genetic, physiological and environmental factors. In order to elucidate their genetic determinism we carried out a quantitative analysis in a 163 individuals-F1 segregating progeny obtained by crossing two table grape cultivars.

ResultsMolecular linkage maps covering most of the genome 2n = 38 for Vitis vinifera were generated for each parent. Eighteen pairs of homologous groups were integrated into a consensus map spanning over 1426 cM with 341 markers mainly microsatellite, AFLP and EST-derived markers and an average map distance between loci of 4.2 cM. Segregating traits were evaluated in three growing seasons by recording flowering, veraison and ripening dates and by measuring berry size, seed number and weight. QTL Quantitative Trait Loci analysis was carried out based on single marker and interval mapping methods. QTLs were identified for all but one of the studied traits, a number of them steadily over more than one year. Clusters of QTLs for different characters were detected, suggesting linkage or pleiotropic effects of loci, as well as regions affecting specific traits. The most interesting QTLs were investigated at the gene level through a bioinformatic analysis of the underlying Pinot noir genomic sequence.

ConclusionOur results revealed novel insights into the genetic control of relevant grapevine features. They provide a basis for performing marker-assisted selection and testing the role of specific genes in trait variation.

List of abbreviations% SDMpercentage of seed dry matter

AFLPAmplified Fragment Length Polymorphism


ERendoplasmic reticulum

ESTExpressed Sequence Tag

F-Rflowering-ripening interval

FTflowering time

F-Vflowering-veraison interval

GAgibberellic acid

LGlinkage group

LODlogarithm of odds

MBWmean berry weight

MQMmultiple QTL mapping

MSDWmean seed dry weight

MSFWmean seed fresh weight

MSNmean seed number


QTLQuantitative Trait Locus

Rripening date

SCARSequence Characterized Amplified Region

SSCPSingle Strand Conformation Polymorphism

SSRSimple Sequence Repeat

VPveraison period

V-Rveraison-ripening interval

VTveraison time.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2229-8-38 contains supplementary material, which is available to authorized users.

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Autor: Laura Costantini - Juri Battilana - Flutura Lamaj - Girolamo Fanizza - Maria Stella Grando


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