The complete genome, comparative and functional analysis of Stenotrophomonas maltophiliareveals an organism heavily shielded by drug resistance determinantsReportar como inadecuado




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Genome Biology

, 9:R74

First Online: 17 April 2008Received: 14 January 2008Revised: 13 March 2008Accepted: 17 April 2008

Abstract

BackgroundStenotrophomonas maltophilia is a nosocomial opportunistic pathogen of the Xanthomonadaceae. The organism has been isolated from both clinical and soil environments in addition to the sputum of cystic fibrosis patients and the immunocompromised. Whilst relatively distant phylogenetically, the closest sequenced relatives of S. maltophilia are the plant pathogenic xanthomonads.

ResultsThe genome of the bacteremia-associated isolate S. maltophilia K279a is 4,851,126 bp and of high G+C content. The sequence reveals an organism with a remarkable capacity for drug and heavy metal resistance. In addition to a number of genes conferring resistance to antimicrobial drugs of different classes via alternative mechanisms, nine resistance-nodulation-division RND-type putative antimicrobial efflux systems are present. Functional genomic analysis confirms a role in drug resistance for several of the novel RND efflux pumps. S. maltophilia possesses potentially mobile regions of DNA and encodes a number of pili and fimbriae likely to be involved in adhesion and biofilm formation that may also contribute to increased antimicrobial drug resistance.

ConclusionThe panoply of antimicrobial drug resistance genes and mobile genetic elements found suggests that the organism can act as a reservoir of antimicrobial drug resistance determinants in a clinical environment, which is an issue of considerable concern.

AbbreviationsACTArtemis Comparison Tool

BSACBritish Society for Antimicrobial Chemotherapy

CDScoding sequence

CFcystic fibrosis

ISinsertion sequence

MDRmulti-drug resistant

MFPmembrane fusion protein

MICminimum inhibitory concentration

N-AHLn-acyl homoserine lactone

OMPouter membrane protein

RNDresistance-nodulation-division.

Electronic supplementary materialThe online version of this article doi:10.1186-gb-2008-9-4-r74 contains supplementary material, which is available to authorized users.

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Autor: Lisa C Crossman - Virginia C Gould - J Maxwell Dow - Georgios S Vernikos - Aki Okazaki - Mohammed Sebaihia - David Saun

Fuente: https://link.springer.com/







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