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BMC Plant Biology

, 8:39

First Online: 17 April 2008Received: 09 July 2007Accepted: 17 April 2008

Abstract

BackgroundBy definition, amyloplasts are plastids specialized for starch production. However, a proteomic study of amyloplasts isolated from wheat Triticum aestivum Butte 86 endosperm at 10 days after anthesis DPA detected enzymes from many other metabolic and biosynthetic pathways. To better understand the role of amyloplasts in food production, the data from that study were evaluated in detail and an amyloplast metabolic map was outlined.

ResultsAnalysis of 288 proteins detected in an amyloplast preparation predicted that 178 were amyloplast proteins. Criteria included homology with known plastid proteins, prediction of a plastid transit peptide for the wheat gene product or a close homolog, known plastid location of the pathway, and predicted plastid location for other members of the same pathway. Of these, 135 enzymes were arranged into 18 pathways for carbohydrate, lipid, amino acid, nucleic acid and other biosynthetic processes that are critical for grain-fill. Functions of the other proteins are also discussed.

ConclusionThe pathways outlined in this paper suggest that amyloplasts play a central role in endosperm metabolism. The interacting effects of genetics and environment on starch and protein production may be mediated in part by regulatory mechanisms within this organelle.

AbbreviationsDPAdays after anthesis.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2229-8-39 contains supplementary material, which is available to authorized users.

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Autor: Frances M Dupont

Fuente: https://link.springer.com/



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