Analysis of skeletal muscle function in the C57BL6-SV129 syncoilin knockout mouseReportar como inadecuado




Analysis of skeletal muscle function in the C57BL6-SV129 syncoilin knockout mouse - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Mammalian Genome

, Volume 19, Issue 5, pp 339–351

First Online: 02 July 2008Received: 21 April 2008Accepted: 28 May 2008

Abstract

Syncoilin is a 64-kDa intermediate filament protein expressed in skeletal muscle and enriched at the perinucleus, sarcolemma, and myotendinous and neuromuscular junctions. Due to its pattern of cellular localization and binding partners, syncoilin is an ideal candidate to be both an important structural component of myocytes and a potential mediator of inherited myopathies. Here we present a report of a knockout mouse model for syncoilin and the results of an investigation into the effect of a syncoilin null state on striated muscle function in 6–8-week-old mice. An analysis of proteins known to associate with syncoilin showed that ablation of syncoilin had no effect on absolute expression or spatial localization of desmin or alpha dystrobrevin. Our syncoilin-null animal exhibited no differences in cardiotoxin-induced muscle regeneration, voluntary wheel running, or enforced treadmill exercise capacity, relative to wild-type controls. Finally, a mechanical investigation of isolated soleus and extensor digitorum longus indicated a potential differential reduction in muscle strength and resilience. We are the first to present data identifying an increased susceptibility to muscle damage in response to an extended forced exercise regime in syncoilin-deficient muscle. This study establishes a second viable syncoilin knockout model and highlights the importance of further investigations to determine the role of syncoilin in skeletal muscle.

K. J. A. McCullagh, B. Edwards, and M. W. Kemp contributed equally to this work.

Download fulltext PDF



Autor: Karl J. A. McCullagh - Ben Edwards - Matthew W. Kemp - Laura C. Giles - Matthew Burgess - Kay E. Davies

Fuente: https://link.springer.com/







Documentos relacionados