The microRNA regulated SBP-box genes SPL9 and SPL15 control shoot maturation in ArabidopsisReportar como inadecuado

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Plant Molecular Biology

, Volume 67, Issue 1–2, pp 183–195

First Online: 17 February 2008Received: 05 July 2007Accepted: 05 February 2008


Throughout development the Arabidopsis shoot apical meristem successively undergoes several major phase transitions such as the juvenile-to-adult and floral transitions until, finally, it will produce flowers instead of leaves and shoots. Members of the Arabidopsis SBP-box gene family of transcription factors have been implicated in promoting the floral transition in dependence of miR156 and, accordingly, transgenics constitutively over-expressing this microRNA are delayed in flowering. To elaborate their roles in Arabidopsis shoot development, we analysed two of the 11 miR156 regulated Arabidopsis SBP-box genes, i.e. the likely paralogous genes SPL9 and SPL15. Single and double mutant phenotype analysis showed these genes to act redundantly in controlling the juvenile-to-adult phase transition. In addition, their loss-of-function results in a shortened plastochron during vegetative growth, altered inflorescence architecture and enhanced branching. In these aspects, the double mutant partly phenocopies constitutive MIR156b over-expressing transgenic plants and thus a major contribution to the phenotype of these transgenics as a result of the repression of SPL9 and SPL15 is strongly suggested.

KeywordsArabidopsis Juvenile phase miRNA Phase change SBP-box genes Shoot maturation AbbreviationsDASDays after sowing

GAGibberellic acid

LDLong day


qRT-PCRQuantitative real-time PCR

SAMShoot apical meristem


SDShort day


Electronic supplementary materialThe online version of this article doi:10.1007-s11103-008-9310-z contains supplementary material, which is available to authorized users.

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Autor: Stefan Schwarz - Arne V. Grande - Nora Bujdoso - Heinz Saedler - Peter Huijser


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