Molecular analysis of enrichment cultures of ammonia oxidizers from the Salar de Huasco, a high altitude saline wetland in northern ChileReportar como inadecuado




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Extremophiles

, Volume 12, Issue 3, pp 405–414

First Online: 28 February 2008Received: 28 May 2007Accepted: 22 January 2008

Abstract

We analyzed enrichment cultures of ammonia-oxidizing bacteria AOB collected from different areas of Salar de Huasco, a high altitude, saline, pH-neutral water body in the Chilean Altiplano. Samples were inoculated into mineral media with 10 mM NH4 at five different salt concentrations 10, 200, 400, 800 and 1,400 mM NaCl. Low diversity up to three phylotypes per enrichment of beta-AOB was detected using 16S rDNA and amoA clone libraries. Growth of beta-AOB was only recorded in a few enrichment cultures and varied according to site or media salinity. In total, five 16S rDNA and amoA phylotypes were found which were related to Nitrosomonas europaea-Nitrosococcus mobilis, N. marina and N. communis clusters. Phylotype 1-16S was 97% similar with N. halophila, previously isolated from Mongolian soda lakes, and phylotypes from amoA sequences were similar with yet uncultured beta-AOB from different biofilms. Sequences related to N. halophila were frequently found at all salinities. Neither gamma-AOB nor ammonia-oxidizing Archaea were recorded in these enrichment cultures.

KeywordsAmmonia-oxidizing bacteria 16S rDNA amoA gene Andean Altiplano Salinity Communicated by K. Horikoshi.

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Autor: Cristina Dorador - Annika Busekow - Irma Vila - Johannes F. Imhoff - Karl-Paul Witzel

Fuente: https://link.springer.com/



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