Characterization of APOBEC3G binding to 7SL RNAReportar como inadecuado




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Retrovirology

, 5:54

First Online: 02 July 2008Received: 23 October 2007Accepted: 02 July 2008

Abstract

Human APOBEC3 proteins are editing enzymes that can interfere with the replication of exogenous retroviruses such as human immunodeficiency virus HIV, hepadnaviruses such as hepatitis B virus HBV, and with the retrotransposition of endogenous retroelements such as long-interspersed nuclear elements LINE and Alu. Here, we show that APOBEC3G, but not other APOBEC3 family members, binds 7SL RNA, the common ancestor of Alu RNAs that is specifically recruited into HIV virions. Our data further indicate that APOBEC3G recognizes 7SL RNA and Alu RNA by its common structure, the Alu domain, suggesting a mechanism for APOBEC3G- mediated inhibition of Alu retrotransposition. However, we also demonstrate that APOBEC3F and APOBEC3G are normally recruited into and inhibit the infectivity of ΔVif HIV1 virions when 7SLRNA is prevented from accessing particles by RNA interference against SRP14 or by over expression of SRP19, both components of the signal recognition particle. We thus conclude that 7SL RNA is not an essential mediator of the virion packaging of these antiviral cytidine deaminases.

Electronic supplementary materialThe online version of this article doi:10.1186-1742-4690-5-54 contains supplementary material, which is available to authorized users.

Daniel Bach, Shyam Peddi contributed equally to this work.

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Autor: Daniel Bach - Shyam Peddi - Bastien Mangeat - Asvin Lakkaraju - Katharina Strub - Didier Trono

Fuente: https://link.springer.com/



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