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Chemistry Central Journal

, 2:14

Analytical Chemistry

Abstract

BackgroundA routine method for the quantification of beryllium in biological fluids is essential for the development of a chelation therapy for Chronic Beryllium Disease CBD. We describe a procedure for the direct determination of beryllium in undigested micro quantities of human blood and serum using graphite furnace atomic absorption spectrometry. Blood and serum samples are prepared respectively by a simple 8-fold and 5-fold dilution with a Nash Reagent. Three experimental setups are compared: using no modifier, using magnesium nitrate and using palladium-citric acid as chemical modifiers.

ResultsIn serum, both modifiers did not improve the method sensitivity, the optimal pyrolysis and atomization temperatures are 1000°C and 2900°C, respectively. In blood, 6 μg of magnesium nitrate was found to improve the method sensitivity. The optimal pyrolysis and atomization temperatures were 800°C and 2800°C respectively.

ConclusionIn serum, the method detection limit was 2 ng l, the characteristic mass was 0.22 ± 0.07 pg and the accuracy ranged from 95 to 100%. In blood, the detection limit was 7 ng l, the characteristic mass was 0.20 ± 0.02 pg and the accuracy ranged from 99 to 101%.

Open image in new windowElectronic supplementary materialThe online version of this article doi:10.1186-1752-153X-2-14 contains supplementary material, which is available to authorized users.

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Autor: Chadi H Stephan - Michel Fournier - Pauline Brousseau - Sébastien Sauvé

Fuente: https://link.springer.com/



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