Prevalence of the prion protein gene E211K variant in U.S. cattleReportar como inadecuado

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BMC Veterinary Research

, 4:25

First Online: 14 July 2008Received: 21 February 2008Accepted: 14 July 2008


BackgroundIn 2006, an atypical U.S. case of bovine spongiform encephalopathy BSE was discovered in Alabama and later reported to be polymorphic for glutamate E and lysine K codons at position 211 in the bovine prion protein gene Prnp coding sequence. A bovine E211K mutation is important because it is analogous to the most common pathogenic mutation in humans E200K which causes hereditary Creutzfeldt – Jakob disease, an autosomal dominant form of prion disease. The present report describes a high-throughput matrix-associated laser desorption-ionization-time-of-flight mass spectrometry assay for scoring the Prnp E211K variant and its use to determine an upper limit for the K211 allele frequency in U.S. cattle.

ResultsThe K211 allele was not detected in 6062 cattle, including those from five commercial beef processing plants 3892 carcasses and 2170 registered cattle from 42 breeds. Multiple nearby polymorphisms in Prnp coding sequence of 1456 diverse purebred cattle 42 breeds did not interfere with scoring E211 or K211 alleles. Based on these results, the upper bounds for prevalence of the E211K variant was estimated to be extremely low, less than 1 in 2000 cattle Bayesian analysis based on 95% quantile of the posterior distribution with a uniform prior.

ConclusionNo groups or breeds of U.S. cattle are presently known to harbor the Prnp K211 allele. Because a carrier was not detected, the number of additional atypical BSE cases with K211 will also be vanishingly low.

Electronic supplementary materialThe online version of this article doi:10.1186-1746-6148-4-25 contains supplementary material, which is available to authorized users.

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Autor: Michael P Heaton - John W Keele - Gregory P Harhay - Jürgen A Richt - Mohammad Koohmaraie - Tommy L Wheeler - Steven 


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