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Nutrition and Metabolism

, 5:19

First Online: 14 July 2008Received: 29 January 2008Accepted: 14 July 2008


BackgroundIt is increasingly recognized that infectious complications in patients treated with total parenteral nutrition TPN may be caused by altered immune responses. Neutrophils and monocytes are the first line of defence against bacterial and fungal infection through superoxide anion production during the respiratory burst. To characterize the impact of three different types of lipid solutions that are applied as part of TPN formulations, we investigated the unstimulated respiratory burst activation of neutrophils and monocytes in whole blood.

MethodsWhole blood samples were incubated with LCT Intralipid, LCT-MCT Lipofundin and LCT-MUFA ClinOleic in three concentrations 0.06, 0.3 and 0.6 mg ml for time periods up to one hour. Hydrogen peroxide production during the respiratory burst of neutrophils and monocytes was measured by flow cytometry.

ResultsLCT and LCT-MUFA induced a hydrogen peroxide production in neutrophils and monocytes without presence of a physiological stimulus in contrast to LCT-MCT.

ConclusionWe concluded that parenteral nutrition containing unsaturated oleic C18:1 and linoleic C18:2 acid can induce respiratory burst of neutrophils and monocytes, resulting in an elevated risk of tissue damage by the uncontrolled production of reactive oxygen species. Contradictory observations reported in previous studies may in part be the result of different methods used to determine hydrogen peroxide production.

Electronic supplementary materialThe online version of this article doi:10.1186-1743-7075-5-19 contains supplementary material, which is available to authorized users.

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Autor: Björn Jüttner - Janina Kröplin - Sina M Coldewey - Lars Witt - Wilhelm A Osthaus - Christian Weilbach - Dirk Scheinich

Fuente: https://link.springer.com/

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