The intrinsic load-resisting capacity of kinesin - Physics > Biological PhysicsReportar como inadecuado




The intrinsic load-resisting capacity of kinesin - Physics > Biological Physics - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Abstract: Conventional kinesin is a homodimeric motor protein that is capable ofwalking unidirectionally along a cytoskeletal filament. While previousexperiments indicated unyielding unidirectionality against an opposing load upto the so-called stall force, recent experiments also observed limitedprocessive backwalking under superstall loads. This theoretical study seeks toelucidate the molecular mechanical basis for kinesin-s steps over the fullrange of external loads that can possibly be applied to the dimer. We foundthat kinesin-s load-resisting capacity is largely determined by a synergicratchet-and-pawl mechanism inherent in the dimer. Load susceptibility of thisinner molecular mechanical mechanism underlies kinesin-s response to variouslevels of external loads. Computational implementation of the mechanism enabledus to rationalize major trends observed experimentally in kinesin-s stalemateand consecutive back steps. The study also predicts several distinct featuresof kinesin-s load-affected motility, which are seemingly counterintuitive butreadily verifiable by future experiment.



Autor: Wenwei Zheng, Dagong Fan, Min Feng, ZhisongWang

Fuente: https://arxiv.org/







Documentos relacionados