Differential effects of class I isoform histone deacetylase depletion and enzymatic inhibition by belinostat or valproic acid in HeLa cellsReportar como inadecuado




Differential effects of class I isoform histone deacetylase depletion and enzymatic inhibition by belinostat or valproic acid in HeLa cells - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Molecular Cancer

, 7:70

First Online: 12 September 2008Received: 30 November 2007Accepted: 12 September 2008

Abstract

BackgroundHistone acetylation is an epigenetic modification involved in the regulation of gene expression, balanced by histone acetyl transferases and histone deacetylase HDAC enzymes. HDAC inhibitors HDACi induce growth arrest and cell death in transformed cells, and are currently in many clinical cancer trials. The transcriptional response to HDACi is complex, as is the response to HDAC isoform knockdown KD. Here, we describe for the first time in a human cancer cell line, a transcriptional comparison of treatment by two structurally unrelated HDACi; belinostat and valproic acid with the KD of HDAC1, 2 and 3 isoforms.

ResultsHDAC KD showed anti-proliferative effects, although to a lesser extent than HDACi treatment. Moreover, we found a 2-fold increased resistance of HDAC1 knockdown cells to belinostat, suggesting this isoenzyme as a selective target. While both HDACi treatment and individual class I HDAC KD produce significant transcriptional effects, three-times higher for HDACi, the gene-expression profiles of class I HDAC KD compared with that obtained by HDACi treatment exhibited less than 4% of altered genes in common between the two modes of inhibition. Further, cell-specific effects of HDAC KD are evident by comparison with a recent study in a different cell line.

ConclusionThe increased resistance to belinostat in response to HDAC1 depletion indicates the possibility of using this isoform as a predictive biomarker of response to HDACi treatment. Further, the transcriptional response to chemical inhibition of HDACs is very different from that of KD of individual class I HDAC isoforms. These data suggest that the anti-tumor effect of HDACi is indeed linked to class I inhibition, but may be more complex than simply targeting individual HDAC enzymes.

List of abbreviationsHDAChistone deacetylase

HDACihistone deacetylase inhibitor

IC50half maximal inhibitory concentration

KDknockdown

qRT-PCRquantitative reverse transcriptase polymerase chain reaction

siRNAsmall interfering ribonucleic acid

TSAtrichostatin A

VPAvalproic acid.

Electronic supplementary materialThe online version of this article doi:10.1186-1476-4598-7-70 contains supplementary material, which is available to authorized users.

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Autor: Marielle Dejligbjerg - Morten Grauslund - Thomas Litman - Laura Collins - Xiaozhong Qian - Michael Jeffers - Henri Lichenst

Fuente: https://link.springer.com/



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