The fungus Neurospora crassa displays telomeric silencing mediated by multiple sirtuins and by methylation of histone H3 lysine 9Reportar como inadecuado




The fungus Neurospora crassa displays telomeric silencing mediated by multiple sirtuins and by methylation of histone H3 lysine 9 - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Epigenetics and Chromatin

, 1:5

First Online: 03 November 2008Received: 14 July 2008Accepted: 03 November 2008

Abstract

BackgroundSilencing of genes inserted near telomeres provides a model to investigate the function of heterochromatin. We initiated a study of telomeric silencing in Neurospora crassa, a fungus that sports DNA methylation, unlike most other organisms in which telomeric silencing has been characterized.

ResultsThe selectable marker, hph, was inserted at the subtelomere of Linkage Group VR in an nst-1 neurospora sir two-1 mutant and was silenced when nst-1 function was restored. We show that NST-1 is an H4-specific histone deacetylase. A second marker, bar, tested at two other subtelomeres, was similarly sensitive to nst-1 function. Mutation of three additional SIR2 homologues, nst-2, nst-3 and nst-5, partially relieved silencing. Two genes showed stronger effects: dim-5, which encodes a histone H3 K9 methyltransferase and hpo, which encodes heterochromatin protein-1. Subtelomeres showed variable, but generally low, levels of DNA methylation. Elimination of DNA methylation caused partial derepression of one telomeric marker. Characterization of histone modifications at subtelomeric regions revealed H3 trimethyl-K9, H3 trimethyl-K27, and H4 trimethyl-K20 enrichment. These modifications were slightly reduced when telomeric silencing was compromised. In contrast, acetylation of histones H3 and H4 increased.

ConclusionWe demonstrate the presence of telomeric silencing in Neurospora and show a dependence on histone deacetylases and methylation of histone H3 lysine 9. Our studies also reveal silencing functions for DIM-5 and HP1 that appear independent of their role in de novo DNA methylation.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-8935-1-5 contains supplementary material, which is available to authorized users.

Kristina M Smith, Gregory O Kothe contributed equally to this work.

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Autor: Kristina M Smith - Gregory O Kothe - Cindy B Matsen - Tamir K Khlafallah - Keyur K Adhvaryu - Melissa Hemphill - Michae

Fuente: https://link.springer.com/







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