Evidence for conserved DNA and histone H3 methylation reprogramming in mouse, bovine and rabbit zygotesReportar como inadecuado




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Epigenetics and Chromatin

, 1:8

First Online: 03 November 2008Received: 26 June 2008Accepted: 03 November 2008

Abstract

BackgroundIn mammals the parental genomes are epigenetically reprogrammed after fertilization. This reprogramming includes a rapid demethylation of the paternal sperm-derived chromosomes prior to DNA replication in zygotes. Such active DNA demethylation in the zygote has been documented for several mammalian species, including mouse, rat, pig, human and cow, but questioned to occur in rabbit.

ResultsWhen comparing immunohistochemical patterns of antibodies against 5-methyl-cytosine, H3K4me3 and H3K9me2 modifications we observe similar pronuclear distribution and dynamics in mouse, bovine and rabbit zygotes. In rabbit DNA demethylation of the paternal chromosomes occurs at slightly advanced pronuclear stages. We also show that the rabbit oocyte rapidly demethylates DNA of donor fibroblast after nuclear transfer.

ConclusionOur data reveal that major events of epigenetic reprogramming during pronuclear maturation, including mechanisms of active DNA demethylation, are apparently conserved among mammalian species.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-8935-1-8 contains supplementary material, which is available to authorized users.

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Autor: Konstantin Lepikhov - Valeri Zakhartchenko - Ru Hao - Feikun Yang - Christine Wrenzycki - Heiner Niemann - Eckhard Wolf - J

Fuente: https://link.springer.com/







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