Inhibition of hepatocelluar carcinoma MAT2A and MAT2beta gene expressions by single and dual small interfering RNAReportar como inadecuado




Inhibition of hepatocelluar carcinoma MAT2A and MAT2beta gene expressions by single and dual small interfering RNA - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Experimental and Clinical Cancer Research

, 27:72

First Online: 21 November 2008Received: 29 July 2008Accepted: 21 November 2008

Abstract

RNA interference RNAi has been successfully applied in suppression of hepatic cancer genes. In hepatocelluar carcinoma cell, one methionine adenosyltransferase MAT isozyme, MATII was found to have two catalytic subunits which were encoded by MAT2A and MAT2β respectively. During tumorigeness of hepatocelluar carcinoma, expressions of the two genes were discovered to be increased combining with a switch of MAT form MATI to MATII, To figure out the role played by MATII in hepatic cancer, In this study, for the first time we established a dual small interfering RNA siRNA expression system, which could simultaneously express two different siRNA molecules specifically targeting two genes. To test the effectiveness of this system, we applied this approach to express simultaneously two different siRNA duplexes that specifically target MAT2A and MAT2β genes of hepatocelluar carcinoma respectively in HepG2 cell. Results indicated that dual siRNA could simultaneously inhibit the expression of MAT2A and MAT2β gene by 89.5% and 97.8% respectively, In addition, dual siRNA molecules were able to significantly suppress growth of hepatocelluar carcinoma cell in vitro as well as induce apoptosis which was involved in arrest cell cycle at the G1-S checkpoint and the expressions of p21, p27 and Bax.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-9966-27-72 contains supplementary material, which is available to authorized users.

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Autor: Qun Wang - Quan-yan Liu - Zhi-Su Liu - Qun Qian - Quan Sun - Ding-yu Pan

Fuente: https://link.springer.com/







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