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Malaria Journal

, 7:241

First Online: 21 November 2008Received: 04 October 2008Accepted: 21 November 2008

Abstract

BackgroundIndividuals living in areas of high malaria transmission often have different Plasmodium falciparum clones detected in the peripheral blood over time. The aim of this study was to assess the dynamics of asymptomatic P. falciparum infections in a few hours intervals.

MethodsCapillary blood samples were collected 6-hourly during five days from asymptomatic children in a highly endemic area in Tanzania. Parasite densities and maturation stages were investigated by light microscopy. Types and number of clones were analysed by PCR based genotyping of the polymorphic merozoite surface proteins 1 and 2 genes. Results: Parasite densities and maturation stages fluctuated 48-hourly with a gradual shift into more mature forms. Various genotyping patterns were observed in repeated samples over five days with only few samples with identical profiles. Up to six alleles differed in samples collected six hours apart in the same individual.

ConclusionThis detailed assessment highlights the extensive within-host dynamics of P. falciparum populations and the limitations of single blood samples to determine parasite densities, stages and genotyping profiles in a malaria infected individual.

List of abbreviationsEDTAethylenediamine-tetraacetic acid

msp1merozoite surface protein 1 gene

msp2merozoite surface protein 2 gene

PCRpolymerase chain reaction

SDSsodium duodecyl sulfate

UVultra violet light.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2875-7-241 contains supplementary material, which is available to authorized users.

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Autor: Anna Färnert - Marianne Lebbad - Lea Faraja - Ingegerd Rooth

Fuente: https://link.springer.com/







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