Diets high in selenium and isoflavones decrease androgen-regulated gene expression in healthy rat dorsolateral prostateReportar como inadecuado

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Reproductive Biology and Endocrinology

, 6:57

First Online: 24 November 2008Received: 10 September 2008Accepted: 24 November 2008


BackgroundHigh dietary intake of selenium or soybean isoflavones reduces prostate cancer risk. These components each affect androgen-regulated gene expression. The objective of this work was to determine the combined effects of selenium and isoflavones on androgen-regulated gene expression in rat prostate.

MethodsMale Noble rats were exposed from conception until 200 days of age to diets containing an adequate 0.33-0.45 mg-kg diet or high 3.33-3.45 mg-kg concentration of selenium as Se-methylselenocysteine and a low 10 mg-kg or high 600 mg-kg level of isoflavones in a 2 × 2 factorial design. Gene expression in the dorsolateral prostate was determined for the androgen receptor, for androgen-regulated genes, and for Akr1c9, whose product catalyzes the reduction of dihydrotestosterone to 5alpha-androstane-3alpha, 17beta-diol. Activity of hepatic glutathione peroxidise 1 and of prostatic 5alpha reductase were also assayed.

ResultsThere were no differences due to diet in activity of liver glutathione peroxidase activity. Total activity of 5alpha reductase in prostate was significantly lower p = 0.007 in rats fed high selenium-high isoflavones than in rats consuming adequate selenium-low isoflavones. High selenium intake reduced expression of the androgen receptor, Dhcr24 24-dehydrocholesterol reductase, and Abcc4 ATP-binding cassette sub-family C member 4. High isoflavone intake decreased expression of Facl3 fatty acid CoA ligase 3, Gucy1a3 guanylate cyclase alpha 3, and Akr1c9. For Abcc4 the combination of high selenium-high isoflavones had a greater inhibitory effect than either treatment alone. The effects of selenium on gene expression were always in the direction of chemoprevention

ConclusionThese results suggest that combined intake of high selenium and high isoflavones may achieve a greater chemopreventive effect than either compound supplemented individually.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-7827-6-57 contains supplementary material, which is available to authorized users.

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Autor: Russell L Legg - Jessica R Tolman - Cameron T Lovinger - Edwin D Lephart - Kenneth DR Setchell - Merrill J Christensen


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