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BMC Complementary and Alternative Medicine

, 8:62

First Online: 06 December 2008Received: 18 September 2008Accepted: 06 December 2008


BackgroundMosquitoes are associated with the transmission of malaria, dengue, Japanese encephalitis, filariasis and other viral diseases throughout the globe, apart from being a nuisance pest. Biological control alone or as a part of integrated vector management stands to be a better alternative to the chemical controls aimed against pest mosquitoes. At the same time it is necessary to control bacteria by synthetic or natural means plant products. Hence the present study was designed to screen the effect of mosquito larvicidal and antimicrobial activitiy of protein isolated from matured leaves of Solanum villosum against mosquito immatures and some pathogenic bacteria.

MethodsAqueous solvent extract of fresh mature leaves of S. villosum was tested against 3rd instar larvae of Anopheles stephensi, Culex quinquefasciatus and Stegomyia aegypti mosquitoes and against four pathogenic bacteria. The protein fraction was isolated and tested for mosquitocidal and antibacterial activities. Amino acid analysis was performed on isolated protein using PICO.TAG amino acid system. SDS-PAGE was also done to detect the bands of amino acid on the basis of their molecular weights.

ResultsProteins isolated from mature leaves of S. villosum were found to have larvicidal and antimicrobial properties. Analysis of the isolated protein identified fifteen amino acids of which eight were essential amino acids. SDS-PAGE detected seven bands corresponding to different molecular weights in the range of 69–109 KDa.

ConclusionProteins of mature leaves of S. villosum exhibited moderate larvicidal and antimicrobial activities. The study provides considerable scope in exploiting local indigenous resources for isolation of antimicrobial and mosquito larvicidal proteins.

Nandita Chowdhury, Subrata Laskar and Goutam Chandra contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6882-8-62 contains supplementary material, which is available to authorized users.

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Autor: Nandita Chowdhury - Subrata Laskar - Goutam Chandra


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