The construction of a Solanum habrochaites LYC4 introgression line population and the identification of QTLs for resistance to Botrytis cinereaReportar como inadecuado




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Theoretical and Applied Genetics

, Volume 114, Issue 6, pp 1071–1080

First Online: 02 February 2007Received: 12 October 2006Accepted: 22 December 2006

Abstract

Tomato Solanum lycopersicum is susceptible to grey mold Botrytis cinerea. Partial resistance to this fungus has been identified in accessions of wild relatives of tomato such as Solanum habrochaites LYC4. In a previous F2 mapping study, three QTLs conferring resistance to B. cinerea Rbcq1, Rbcq2 and Rbcq4a were identified. As it was probable that this study had not identified all QTLs involved in resistance we developed an introgression line IL population n = 30, each containing a S. habrochaites introgression in the S. lycopersicum cv. Moneymaker genetic background. On average each IL contained 5.2% of the S. habrochaites genome and together the lines provide an estimated coverage of 95%. The level of susceptibility to B. cinerea for each of the ILs was assessed in a greenhouse trial and compared to the susceptible parent S. lycopersicum cv. Moneymaker. The effect of the three previously identified loci could be confirmed and seven additional loci were detected. Some ILs contains multiple QTLs and the increased resistance to B. cinerea in these ILs is in line with a completely additive model. We conclude that this set of QTLs offers good perspectives for breeding of B. cinerea resistant cultivars and that screening an IL population is more sensitive for detection of QTLs conferring resistance to B. cinerea than the analysis in an F2 population.

Communicated by R. Waugh.

Electronic supplementary materialThe online version of this article doi:10.1007-s00122-006-0500-2 contains supplementary material, which is available to authorized users.

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Autor: Richard Finkers - Adriaan W. van Heusden - Fien Meijer-Dekens - Jan A. L. van Kan - Paul Maris - Pim Lindhout

Fuente: https://link.springer.com/







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