X-ray structures of Na-GST-1 and Na-GST-2 two glutathione s-transferase from the human hookworm Necator americanusReportar como inadecuado

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BMC Structural Biology

, 7:42

First Online: 26 June 2007Received: 09 February 2007Accepted: 26 June 2007


BackgroundHuman hookworm infection is a major cause of anemia and malnutrition of adults and children in the developing world. As part of on-going efforts to control hookworm infection, The Human Hookworm Vaccine Initiative has identified candidate vaccine antigens from the infective L3 larval stages and adult stages of the parasite. Adult stage antigens include the cytosolic glutathione-S-transferases GSTs. Nematode GSTs facilitate the inactivation and degradation of a variety of electrophilic substrates drugs via the nucleophilic addition of reduced glutathione. Parasite GSTs also play significant roles in multi-drug resistance and the modulation of host-immune defense mechanisms.

ResultsThe crystal structures of Na-GST-1 and Na-GST-2, two major GSTs from Necator americanus the main human hookworm parasite, have been solved at the resolution limits of 2.4 Å and 1.9 Å respectively. The structure of Na-GST-1 was refined to R-factor 18.9% R-free 28.3% while that of Na-GST-2 was refined to R-factor 17.1% R-free 21.7%. Glutathione usurped during the fermentation process in bound in the glutathione binding site G-site of each monomer of Na-GST-2. Na-GST-1 is uncomplexed and its G-site is abrogated by Gln 50. These first structures of human hookworm parasite GSTs could aid the design of novel hookworm drugs.

ConclusionThe 3-dimensional structures of Na-GST-1 and Na-GST-2 show two views of human hookworm GSTs. While the GST-complex structure of Na-GST-2 reveals a typical GST G-site that of Na-GST-1 suggests that there is some conformational flexibility required in order to bind the substrate GST. In addition, the overall binding cavities for both are larger, more open, as well as more accessible to diverse ligands than those of GSTs from organisms that have other major detoxifying mechanisms. The results from this study could aid in the design of novel drugs and vaccine antigens.

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6807-7-42 contains supplementary material, which is available to authorized users.

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Autor: Oluwatoyin A Asojo - Kohei Homma - Meghan Sedlacek - Michelle Ngamelue - Gaddam N Goud - Bin Zhan - Vehid Deumic - Oluyom

Fuente: https://link.springer.com/

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