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Respiratory Research

, 8:63

First Online: 14 September 2007Received: 12 April 2007Accepted: 14 September 2007


The widespread use of genetically altered mouse models of experimental asthma has stimulated the development of lung function techniques in vivo to characterize the functional results of genetic manipulations. Here, we describe various classical and recent methods of measuring airway responsiveness in vivo including both invasive methodologies in anesthetized, intubated mice repetitive-non-repetitive assessment of pulmonary resistance RL and dynamic compliance Cdyn; measurement of low-frequency forced oscillations LFOT and noninvasive technologies in conscious animals head-out body plethysmography; barometric whole-body plethysmography. Outlined are the technical principles, validation and applications as well as the strengths and weaknesses of each methodology. Reviewed is the current set of invasive and noninvasive methods of measuring murine pulmonary function, with particular emphasis on practical considerations that should be considered when applying them for phenotyping in the laboratory mouse.

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Autor: Thomas Glaab - Christian Taube - Armin Braun - Wayne Mitzner


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