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Genome Biology

, 8:R192

First Online: 14 September 2007Received: 07 March 2007Revised: 25 July 2007Accepted: 14 September 2007

Abstract

We propose InSite, a computational method that integrates high-throughput protein and sequence data to infer the specific binding regions of interacting protein pairs. We compared our predictions with binding sites in Protein Data Bank and found significantly more binding events occur at sites we predicted. Several regions containing disease-causing mutations or cancer polymorphisms in human are predicted to be binding for protein pairs related to the disease, which suggests novel mechanistic hypotheses for several diseases.

AbbreviationsAUCarea under the ROC curve

EMexpectation maximization

GOGene Ontology

HPRDHuman Protein Reference Database

InSiteInteraction Site

MSmass spectrometry

OMIMOnline Mendelian Inheritance in Man

PDBProtein Data Bank

Pol IIRNA polymerase II

ROCreceiver operating characteristic

TAPtandem affinity purification.

Electronic supplementary materialThe online version of this article doi:10.1186-gb-2007-8-9-r192 contains supplementary material, which is available to authorized users.

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Autor: Haidong Wang - Eran Segal - Asa Ben-Hur - Qian-Ru Li - Marc Vidal - Daphne Koller

Fuente: https://link.springer.com/







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