Inverse association of plasma IL-13 and inflammatory chemokines with lung function impairment in stable COPD: a cross-sectional cohort studyReport as inadecuate

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Respiratory Research

, 8:64

First Online: 14 September 2007Received: 11 May 2007Accepted: 14 September 2007


BackgroundChronic obstructive pulmonary disease COPD is a heterogeneous syndrome characterized by varying degrees of airflow limitation and diffusion impairment. There is increasing evidence to suggest that COPD is also characterized by systemic inflammation. The primary goal of this study was to identify soluble proteins in plasma that associate with the severity of airflow limitation in a COPD cohort with stable disease. A secondary goal was to assess whether unique markers associate with diffusion impairment, based on diffusion capacity of carbon monoxide DLCO, independent of the forced expiratory volume in 1 second FEV1.

MethodsA cross sectional study of 73 COPD subjects was performed in order to examine the association of 25 different plasma proteins with the severity of lung function impairment, as defined by the baseline measurements of the % predicted FEV1 and the % predicted DLCO. Plasma protein concentrations were assayed using multiplexed immunobead-based cytokine profiling. Associations between lung function and protein concentrations were adjusted for age, gender, pack years smoking history, current smoking, inhaled corticosteroid use, systemic corticosteroid use and statin use.

ResultsPlasma concentrations of CCL2-monocyte chemoattractant protein-1 CCL2-MCP-1, CCL4-macrophage inflammatory protein-1β CCL4-MIP -1β, CCL11-eotaxin, and interleukin-13 IL-13 were inversely associated with the % FEV1. Plasma concentrations of soluble Fas were associated with the % DLCO, whereas CXCL9-monokine induced by interferon-γ CXCL9-Mig, granulocyte- colony stimulating factor G-CSF and IL-13 showed inverse relationships with the % DLCO.

ConclusionSystemic inflammation in a COPD cohort is characterized by cytokines implicated in inflammatory cell recruitment and airway remodeling. Plasma concentrations of IL-13 and chemoattractants for monocytes, T lymphocytes, and eosinophils show associations with increasing severity of disease. Soluble Fas, G-CSF and CXCL9-Mig may be unique markers that associate with disease characterized by disproportionate abnormalities in DLCO independent of the FEV1.

AbbreviationsCCL2-MCP-1CC chemokine ligand 2-monocyte chemotattractant protein-1

CCL3-MIP-1αCC chemokine ligand 3-macrophage inflammatory protein-1α

CCL4-MIP-1βCC chemokine ligand 4-macrophage inflammatory protein-1β

CCL5-RANTESCC chemokine ligand 5-regulated on activation normal T cell expressed and secreted

CCL11-eotaxinCC chemokine ligand 11-eotaxin

CRPC-reactive protein

CXCL8-IL-8CXC chemokine ligand 8-interleukin-8

CXCL9-MigCXC chemokine ligand 9-monkine induced by interferon-γ

EGFepidermal growth factor

EGFRepidermal growth factor receptor

FasLFas ligand

FGFβfibroblast growth factor β

G-CSFgranulocyte-colony stimulating factor

HGFhepatocyte growth factor




IL-2Rinterleukin-2 receptor

IL-4interleukin-4, IL-6, interleukin-6




TNF-αtumor necrosis factor α

TNFRItumor necrosis factor receptor 1

TNFRIItumor necrosis factor receptor 2

VEGFvascular endothelial growth factor

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Author: Janet S Lee - Matthew R Rosengart - Venkateswarlu Kondragunta - Yingze Zhang - Jessica McMurray - Robert A Branch - Augus


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