A genetic code alteration generates a proteome of high diversity in the human pathogen Candida albicansReportar como inadecuado

A genetic code alteration generates a proteome of high diversity in the human pathogen Candida albicans - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Genome Biology

, 8:R206

First Online: 04 October 2007Received: 10 May 2007Revised: 31 July 2007Accepted: 04 October 2007


BackgroundGenetic code alterations have been reported in mitochondrial, prokaryotic, and eukaryotic cytoplasmic translation systems, but their evolution and how organisms cope and survive such dramatic genetic events are not understood.

ResultsHere we used an unusual decoding of leucine CUG codons as serine in the main human fungal pathogen Candida albicans to elucidate the global impact of genetic code alterations on the proteome. We show that C. albicans decodes CUG codons ambiguously and tolerates partial reversion of their identity from serine back to leucine on a genome-wide scale.

ConclusionSuch codon ambiguity expands the proteome of this human pathogen exponentially and is used to generate important phenotypic diversity. This study highlights novel features of C. albicans biology and unanticipated roles for codon ambiguity in the evolution of the genetic code.

AbbreviationsANOVAanalysis of variance

bpbase pairs

CAIcodon adaptation index

EFelongation factor

5-FOA5-fluoro-orotic acid

H2O2hydrogen peroxide

HPLChigh-pressure liquid chromatography

Hspheat shock protein

LeuRSleucyl-tRNA synthetase

ORFopen reading frame

PCRpolymerase chain reaction



Electronic supplementary materialThe online version of this article doi:10.1186-gb-2007-8-10-r206 contains supplementary material, which is available to authorized users.

Download fulltext PDF

Autor: Ana C Gomes - Isabel Miranda - Raquel M Silva - Gabriela R Moura - Benjamin Thomas - Alexandre Akoulitchev - Manuel AS 

Fuente: https://link.springer.com/

Documentos relacionados