Posttraumatic secondary brain insults exacerbates neuronal injury by altering Metabotropic Glutamate ReceptorsReportar como inadecuado




Posttraumatic secondary brain insults exacerbates neuronal injury by altering Metabotropic Glutamate Receptors - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Neuroscience

, 8:96

First Online: 17 November 2007Received: 09 May 2007Accepted: 17 November 2007

Abstract

BackgroundOur previous studies indicated that metabotropic glutamate receptors mGluRs are deeply involved in the secondary processes after diffuse brain injury DBI. In the present study, we used a rodent DBI model to determine whether hypotension exacerbates neuronal injury as a secondary brain insult SBI after traumatic brain injury TBI by changing the expression of metabotropic glutamate receptors mGluRs in the cerebral cortex.

ResultsThree hundred and eleven male Sprague-Dawley rats were randomly assigned into five groups: normal control, sham-operated control, SBI alone, DBI alone, or DBI with SBI. DBI was produced in rats by Marmarou-s methods and the SBI model was produced by hypotension. The alteration of neuronal expression of mGluRs after DBI and DBI coupled with SBI was observed by hybridization in situ at different time points in the experiment. We found a higher mortality and neurological severity score NSS for rats in the DBI with SBI group compared with those in the DBI alone group. Although there was a significant rise in the expression of group I and group III mGluRs except mGluR6 and a decrease in the expression of group II mGluRs after DBI P < 0.05, the changes were more severe when DBI was coupled with SBI P < 0.05. The expression of group I mGluRs peaked at 24 hours, while the expression of the group III mGluRs peaked at 6 hours after injuries, which may reflect a self-protection first mechanism of the damaged neurons. Moreover, the overall neuro-harmful effects of mGluRs on neurons were seemly associated with higher mortality and NSS in the DBI with SBI group.

ConclusionThe results suggest posttraumatic SBI may exacerbate neuronal injury or brain injury by altering expression of mGluRs, and more emphasis should therefore be put on the prevention and treatment of SBI.

List of abbreviationsBLASTbasic local alignment search tool

CBFcerebral blood flow

DBIdiffuse brain injury

iGluRsionotropic glutamate receptors

MABPmean arterial blood pressure

mGluRsmetabotropic glutamate receptors

NSSneurological severity score

SBIsecondary brain insults

TBItraumatic brain injury

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2202-8-96 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Zhou Fei - Xiang Zhang - Hong-min Bai - Xiao-fan Jiang - Xia Li - Wei Zhang - Wei Hu

Fuente: https://link.springer.com/



DESCARGAR PDF




Documentos relacionados