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Nanoscale Research Letters

, 2:614

First Online: 17 November 2007Received: 26 June 2007Accepted: 30 October 2007


Bactericidal efficacy of gold nanoparticles conjugated with ampicillin, streptomycin and kanamycin were evaluated. Gold nanoparticles Gnps were conjugated with the antibiotics during the synthesis of nanoparticles utilizing the combined reducing property of antibiotics and sodium borohydride. The conjugation of nanoparticles was confirmed by dynamic light scattering DLS and electron microscopic EM studies. Such Gnps conjugated antibiotics showed greater bactericidal activity in standard agar well diffusion assay. The minimal inhibitory concentration MIC values of all the three antibiotics along with their Gnps conjugated forms were determined in three bacterial strains,Escherichia coli DH5α,Micrococcus luteus andStaphylococcus aureus. Among them, streptomycin and kanamycin showed significant reduction in MIC values in their Gnps conjugated form whereas; Gnps conjugated ampicillin showed slight decrement in the MIC value compared to its free form. On the other hand, all of them showed more heat stability in their Gnps conjugated forms. Thus, our findings indicated that Gnps conjugated antibiotics are more efficient and might have significant therapeutic implications.

KeywordsGold nanoparticles Antibiotics Dynamic light scattering Transmission electron microscope Scanning electron microscope Minimal inhibitory concentration Agar well diffusion  Download fulltext PDF

Autor: Biswarup Saha - Jaydeep Bhattacharya - Ananda Mukherjee - AnupKumar Ghosh - ChittaRanjan Santra - Anjan K Dasgupta - Parima


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