Genetic variability of the P120 surface protein gene of Mycoplasma hominis isolates recovered from Tunisian patients with uro-genital and infertility disordersReportar como inadecuado




Genetic variability of the P120 surface protein gene of Mycoplasma hominis isolates recovered from Tunisian patients with uro-genital and infertility disorders - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Infectious Diseases

, 7:142

First Online: 05 December 2007Received: 28 April 2007Accepted: 05 December 2007

Abstract

BackgroundAmong the surface antigens of Mycoplasma hominis, the P120- protein was previously shown to elicit a subtle antibody response and appears to be relatively conserved. To get better insight into the evolution of this protein, we analysed the genetic variability of its surface exposed region in 27 M. hominis isolates recovered from the genital tract of Tunisian patients with infertility disorders.

MethodsAll specimens were processed for culture and PCR amplification of the N-terminal surface exposed region of p120- gene. PCR products were sequenced to evaluate the genetic variability, to test for adaptive selection, and to infer the phylogenetic relationship of the M. hominis isolates.

ResultsSequence analysis showed a total of 25 single nucleotide polymorphisms distributed through 23 polymorphic sites, yielding 13 haplotypes. All but one mutation were confined within three distinct regions. Analysis of the amino acid-based phylogenetic tree showed a predominant group of 17 closely related isolates while the remaining appear to have significantly diverged.

ConclusionBy analysing a larger sample of M. hominis recovered from patients with urogenital infections, we show here that the P120- protein undergoes substantial level of genetic variability at its surface exposed region.

List of abbreviationsSNPSingle nucleotide polymorphism

sSNPsynonymous single nucleotide polymorphism

nsSNPnonsynonymous single nucleotide polymorphism

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2334-7-142 contains supplementary material, which is available to authorized users.

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Autor: Boutheina Ben Abdelmoumen Mardassi - Hajer Ayari - Awatef Béjaoui-Khiari - Béhija Mlik - Imed Moalla - Faten Amouna

Fuente: https://link.springer.com/







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