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Journal of Nanobiotechnology

, 4:1

First Online: 22 January 2006Received: 11 August 2005Accepted: 22 January 2006

Abstract

A technique for permanently capturing a replica impression of biological cells has been developed to facilitate analysis using nanometer resolution imaging tools, namely the atomic force microscope AFM. The method, termed Bioimprint™, creates a permanent cell -footprint- in a non-biohazardous Poly dimethylsiloxane PDMS polymer composite. The transfer of nanometer scale biological information is presented as an alternative imaging technique at a resolution beyond that of optical microscopy. By transferring cell topology into a rigid medium more suited for AFM imaging, many of the limitations associated with scanning of biological specimens can be overcome. Potential for this technique is demonstrated by analyzing Bioimprint™ replicas created from human endometrial cancer cells. The high resolution transfer of this process is further detailed by imaging membrane morphological structures consistent with exocytosis. The integration of soft lithography to replicate biological materials presents an enhanced method for the study of biological systems at the nanoscale.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-3155-4-1 contains supplementary material, which is available to authorized users.

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Autor: JJ Muys - MM Alkaisi - DOS Melville - J Nagase - P Sykes - GM Parguez - JJ Evans

Fuente: https://link.springer.com/



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