Isolation of avian influenza virus H9N2 from emu in ChinaReportar como inadecuado

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Irish Veterinary Journal

, 59:148

First Online: 01 March 2006


This is the first reported isolation of avian influenza virus AIV from emu in China. An outbreak of AIV infection occurred at an emu farm that housed 40 four-month-old birds. Various degrees of haemorrhage were discovered in the tissues of affected emus. Cell degeneration and necrosis were observed microscopically. Electron microscopy revealed round or oval virions with a diameter of 80 nm to 120 nm, surrounded by an envelope with spikes. The virus was classified as low pathogenic AIV LPAIV, according to OIE standards. It was named A-Emu-HeNen-14-2004H9N2Emu-HN-2004. The HA gene 1683bp was amplified by RT-PCR and it was compared with other animal H9N2 AIV sequences in GenBank, the US National Institutes of Health genetic sequence database. The results suggested that Emu-HN-2004 may have come from an avian influenza virus H9N2 from Southern China.

KeywordsEmu Avian influenza virus H9N2 AbbreviationsAIV avian influenza virus

CPE cell pathogenic effect

HA test haemagglutination activity test

HI test haemagglutination inhibition test

RT-PCR reverse transcription-polymerase chain reaction.

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Autor: Wenhua Kang - Wanyong Pang - Junfeng Hao - Deming Zhao


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