RNA editing of human microRNAsReport as inadecuate

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Genome Biology

, 7:R27

First Online: 04 April 2006Received: 08 December 2005Revised: 30 January 2006Accepted: 06 March 2006


BackgroundMicroRNAs miRNAs are short RNAs of around 22 nucleotides that regulate gene expression. The primary transcripts of miRNAs contain double-stranded RNA and are therefore potential substrates for adenosine to inosine A-to-I RNA editing.

ResultsWe have conducted a survey of RNA editing of miRNAs from ten human tissues by sequence comparison of PCR products derived from matched genomic DNA and total cDNA from the same individual. Six out of 99 6% miRNA transcripts from which data were obtained were subject to A-to-I editing in at least one tissue. Four out of seven edited adenosines were in the mature miRNA and were predicted to change the target sites in 3- untranslated regions. For a further six miRNAs, we identified A-to-I editing of transcripts derived from the opposite strand of the genome to the annotated miRNA. These miRNAs may have been annotated to the wrong genomic strand.

ConclusionOur results indicate that RNA editing increases the diversity of miRNAs and their targets, and hence may modulate miRNA function.

Electronic supplementary materialThe online version of this article doi:10.1186-gb-2006-7-4-r27 contains supplementary material, which is available to authorized users.

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Author: Matthew J Blow - Russell J Grocock - Stijn van Dongen - Anton J Enright - Ed Dicks - P Andrew Futreal - Richard Wooster

Source: https://link.springer.com/

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