The placental cholinergic system: localization to the cytotrophoblast and modulation of nitric oxideReportar como inadecuado

The placental cholinergic system: localization to the cytotrophoblast and modulation of nitric oxide - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Cell Communication and Signaling

, 4:4

First Online: 10 May 2006Received: 23 December 2005Accepted: 10 May 2006


BackgroundThe human placenta, a non-neuronal tissue, contains an active cholinergic system comprised of acetylcholine ACh, choline acetyltransferase ChAT, acetylcholinesterase AChE, and high affinity muscarinic receptors. The cells of origin of placental ACh and its role in trophoblast function has not been defined. These studies were performed to define the cellular location of ACh synthesis ChAT in the human placenta and to begin studying its functional role.

ResultsUsing immunohistochemical techniques, ChAT was observed primarily within the cytotrophoblasts of preterm placentae as well as some mesenchymal elements. Similar intense immunostaining of the cytotrophoblast was observed for endothelium-derived nitric oxide synthase eNOS suggesting that ACh may interact with nitric oxide NO-dependent signaling pathways. The ability of carbamylcholine CCh, an ACh analogue, to stimulate a rise in intracellular Ca and NO production in trophoblasts was therefore tested using the BeWo choriocarcinoma cell as a model system. First, CCh significantly increased intracellular calcium as assessed by fluorescence microscopy. We then examined the ability of CCh to stimulate NO production by measuring total nitrite-nitrate production in conditioned media using chemiluminescence-based analysis. CCh, alone, had no effect on NO production. However, CCh increased measurable NO approximately 100% in the presence of 10 nM estradiol. This stimulatory effect was inhibited by 1 microM scopolamine suggesting mediation via muscarinic receptors. Estradiol, alone, had no effect on total NO or eNOS protein or mRNA.

ConclusionThese data demonstrate that placental ChAT localizes to the cytotrophoblast and some mesenchymal cells in human placenta. It further suggests that ACh acts via muscarinic receptors on the trophoblast cell membrane to modulate NO in an estrogen-dependent manner.

Electronic supplementary materialThe online version of this article doi:10.1186-1478-811X-4-4 contains supplementary material, which is available to authorized users.

Download fulltext PDF

Autor: Md Badiul Bhuiyan - Ferid Murad - Michael E Fant


Documentos relacionados