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BMC Neuroscience

, 7:50

First Online: 19 June 2006Received: 24 March 2006Accepted: 19 June 2006


BackgroundThe LAR family Protein Tyrosine Phosphatase sigma PTPσ has been implicated in neuroendocrine and neuronal development, and shows strong expression in specific regions within the CNS, including the subventricular zone SVZ. We established neural stem cell cultures, grown as neurospheres, from the SVZ of PTPσ knockout mice and sibling controls to determine if PTPσ influences the generation and the phenotype of the neuronal, astrocyte and oligodendrocyte cell lineages.

ResultsThe neurospheres from the knockout mice acquired heterogeneous developmental characteristics and they showed similar morphological characteristics to the age matched siblings. Although Ptprs expression decreases as a function of developmental age in vivo, it remains high with the continual renewal and passage of the neurospheres. Stem cells, progenitors and differentiated neurons, astrocytes and oligodendrocytes all express the gene. While no apparent differences were observed in developing neurospheres or in the astrocytes and oligodendrocytes from the PTPσ knockout mice, the neuronal migration patterns and neurites were altered when studied in culture. In particular, neurons migrated farther from the neurosphere centers and the neurite outgrowth exceeded the length of the neuronal processes from age matched sibling controls.

ConclusionOur results imply a specific role for PTPσ in the neuronal lineage, particularly in the form of inhibitory influences on neurite outgrowth, and demonstrate a role for tyrosine phosphatases in neuronal stem cell differentiation.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2202-7-50 contains supplementary material, which is available to authorized users.

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Autor: David L Kirkham - Laura KK Pacey - Michelle M Axford - Roberta Siu - Daniela Rotin - Laurie C Doering


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