Cysteinyl-leukotrienes in the regulation of β2-adrenoceptor function: an in vitro model of asthmaReportar como inadecuado

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Respiratory Research

, 7:103

First Online: 28 July 2006Received: 31 March 2006Accepted: 28 July 2006


BackgroundThe response to β2-adrenoceptor agonists is reduced in asthmatic airways. This desensitization may be in part due to inflammatory mediators and may involve cysteinyl-leukotrienes cysteinyl-LTs. Cysteinyl-LTs are pivotal inflammatory mediators that play important roles in the pathophysiology of asthma, allergic rhinitis, and other inflammatory conditions. We tested the hypothesis that leukotriene D4 LTD4 and allergen challenge cause β2-adrenoceptor desensitization through the activation of protein kinase C PKC.

MethodsThe isoproterenol-induced cAMP accumulation was evaluated in human airway smooth muscle cell cultures challenged with exogenous LTD4 or the PKC activator phorbol-12-myristate-13-acetate with or without pretreatments with the PKC inhibitor GF109203X or the CysLT1R antagonist montelukast. The relaxant response to salbutamol was studied in passively sensitized human bronchial rings challenged with allergen in physiological salt solution PSS alone, or in the presence of either montelukast or GF109203X.

ResultsIn cell cultures, both LTD4 and phorbol-12-myristate-13-acetate caused significant reductions of maximal isoproterenol-induced cAMP accumulation, which were fully prevented by montelukast and GF109203X, respectively. More importantly, GF109203X also prevented the attenuating effect of LTD4 on isoproterenol-induced cAMP accumulation. In bronchial rings, both montelukast and GF109203X prevented the rightward displacement of the concentration-response curves to salbutamol induced by allergen challenge.

ConclusionLTD4 induces β2-adrenoceptor desensitization in human airway smooth muscle cells, which is mediated through the activation of PKC. Allergen exposure of sensitized human bronchi may also cause a β2-adrenoceptor desensitization through the involvement of the CysLT1R-PKC pathway.

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Autor: G Enrico Rovati - Michele Baroffio - Simona Citro - Lorenzo Brichetto - Saula Ravasi - Manlio Milanese - Emanuele Crimi -


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