Genetic analysis of the GRIK2modifier effect in Huntingtons diseaseReportar como inadecuado

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BMC Neuroscience

, 7:62

First Online: 07 September 2006Received: 17 July 2006Accepted: 07 September 2006


BackgroundIn Huntington-s disease HD, age at neurological onset is inversely correlated with the length of the CAG trinucleotide repeat mutation, but can be modified by genetic factors beyond the HD gene. Association of a relatively infrequent 16 TAA allele of a trinucleotide repeat polymorphism in the GRIK2 3-UTR with earlier than expected age at neurological onset has been suggested to reflect linkage disequilibrium with a functional polymorphism in GRIK2 or an adjacent gene.

ResultsWe have tested this hypothesis by sequencing all GRIK2 exons, the exon-flanking sequences and 3-UTR in several individuals who were crucial to demonstrating the modifier effect, as they showed much earlier age at neurological onset than would be expected from the length of their HD CAG mutation. Though ten known SNPs were detected, no sequence variants were found in coding or adjacent sequence that could explain the modifier effect by linkage disequilibrium with the 16 TAA allele. Haplotype analysis using microsatellites, known SNPs and new variants discovered in the 3-UTR argues against a common ancestral origin for the 16 TAA repeat alleles in these individuals.

ConclusionThese data suggest that the modifier effect is actually due to the TAA repeat itself, possibly via a functional consequence on the GRIK2 mRNA.

AbbreviationsHDHuntington-s disease

UTRuntranslated region.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2202-7-62 contains supplementary material, which is available to authorized users.

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Autor: Wenqi Zeng - Tammy Gillis - Michael Hakky - Luc Djoussé - Richard H Myers - Marcy E MacDonald - James F Gusella


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