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1 IMNC - Imagerie et Modélisation en Neurobiologie et Cancérologie 2 IMIV - Imagerie moléculaire in vivo

Abstract : Optical properties of fresh and frozen tissues of rat heart, kidney, brain, liver, and muscle were measured in the 450-to 700-nm range. The total reflectance and transmittance were measured using a well-calibrated integral sphere setup. Absorption coefficient μ a and reduced scattering coefficient μ 0 s were derived from the experimental measurements using the inverse adding doubling technique. The influence of cryogenic processing on optical properties was studied. Interindividual and intraindividual variations were assessed. These new data aim at filling the lack of validated optical properties in the visible range especially in the blue-green region of particular interest for fluorescence and optogenetics preclinical studies. Furthermore, we provide a unique comparison of the optical properties of different organs obtained using the same measurement setup for fresh and frozen tissues as well as an estimate of the intraindividual and interindividual variability.

Keywords : optical properties Monte Carlo simulations rat heart kidney liver brain muscle Paper 130573R





Autor: Mohammed Mesradi - Aurelie Genoux - Vesna Cuplov - Darine Abi Haidar - Sebastien Jan - Irene Buvat - Frederic Pain -

Fuente: https://hal.archives-ouvertes.fr/



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