Quantitative genomics of starvation stress resistance in DrosophilaReportar como inadecuado




Quantitative genomics of starvation stress resistance in Drosophila - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Genome Biology

, 6:R36

First Online: 24 March 2005Received: 24 August 2004Revised: 22 December 2004Accepted: 23 February 2005

Abstract

BackgroundA major challenge of modern biology is to understand the networks of interacting genes regulating complex traits, and the subset of these genes that affect naturally occurring quantitative genetic variation. Previously, we used P-element mutagenesis and quantitative trait locus QTL mapping in Drosophila to identify candidate genes affecting resistance to starvation stress, and variation in resistance to starvation stress between the Oregon-R Ore and 2b strains. Here, we tested the efficacy of whole-genome transcriptional profiling for identifying genes affecting starvation stress resistance.

ResultsWe evaluated whole-genome transcript abundance for males and females of Ore, 2b, and four recombinant inbred lines derived from them, under control and starved conditions. There were significant differences in transcript abundance between the sexes for nearly 50% of the genome, while the transcriptional response to starvation stress involved approximately 25% of the genome. Nearly 50% of P-element insertions in 160 genes with altered transcript abundance during starvation stress had mutational effects on starvation tolerance. Approximately 5% of the genome exhibited genetic variation in transcript abundance, which was largely attributable to regulation by unlinked genes. Genes exhibiting variation in transcript abundance among lines did not cluster within starvation resistance QTLs, and none of the candidate genes affecting variation in starvation resistance between Ore and 2b exhibited significant differences in transcript abundance between lines.

ConclusionsExpression profiling is a powerful method for identifying networks of pleiotropic genes regulating complex traits, but the relationship between variation in transcript abundance among lines used to map QTLs and genes affecting variation in quantitative traits is complicated.

Electronic supplementary materialThe online version of this article doi:10.1186-gb-2005-6-4-r36 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Susan T Harbison - Sherman Chang - Kim P Kamdar - Trudy FC Mackay

Fuente: https://link.springer.com/



DESCARGAR PDF




Documentos relacionados