Typing of human rotaviruses: Nucleotide mismatches between the VP7 gene and primer are associated with genotyping failureReportar como inadecuado




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Virology Journal

, 2:24

First Online: 24 March 2005Received: 06 March 2005Accepted: 24 March 2005

Abstract

BackgroundRotavirus genotyping is performed by using reverse transcription PCR with type-specific-primers. Because the high rotavirus mutation rate generates an extensive genomic variation, different G-type-specific primer sets are applied in different geographical locations. In Bangladesh, a significant proportion 36.9% of the rotavirus strains isolated in 2002 could not be G-typed using the routinely used primer set. To investigate the reason why the strains were untypeable, nucleotide sequencing of the VP7 genes was performed.

ResultsFour nucleotide substitutions at the G1 primer-binding site of the VP7 gene of Bangladeshi G1 rotaviruses rendered a major proportion of circulating strains untypeable using the routine primer set. Using an alternative primer set, we could identify G1 rotaviruses as the most prevalent genotype 44.8%, followed by G9 21.7%, G2 15.0% and G4 13.8%.

ConclusionBecause of the natural variation in the rotaviral gene sequences, close monitoring of rotavirus genotyping methods is important.

Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-2-24 contains supplementary material, which is available to authorized users.

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Autor: Mustafizur Rahman - Rasheda Sultana - Goutam Podder - Abu SG Faruque - Jelle Matthijnssens - Khalequz Zaman - Robert F Br

Fuente: https://link.springer.com/







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