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BMC Physiology

, 5:5

First Online: 24 March 2005Received: 13 September 2004Accepted: 24 March 2005

Abstract

BackgroundChronic fatigue syndrome CFS is defined by debilitating fatigue that is exacerbated by physical or mental exertion. To search for markers of CFS-associated post-exertional fatigue, we measured peripheral blood gene expression profiles of women with CFS and matched controls before and after exercise challenge.

ResultsWomen with CFS and healthy, age-matched, sedentary controls were exercised on a stationary bicycle at 70% of their predicted maximum workload. Blood was obtained before and after the challenge, total RNA was extracted from mononuclear cells, and signal intensity of the labeled cDNA hybridized to a 3800-gene oligonucleotide microarray was measured. We identified differences in gene expression among and between subject groups before and after exercise challenge and evaluated differences in terms of Gene Ontology categories.

Exercise-responsive genes differed between CFS patients and controls. These were in genes classified in chromatin and nucleosome assembly, cytoplasmic vesicles, membrane transport, and G protein-coupled receptor ontologies. Differences in ion transport and ion channel activity were evident at baseline and were exaggerated after exercise, as evidenced by greater numbers of differentially expressed genes in these molecular functions.

ConclusionThese results highlight the potential use of an exercise challenge combined with microarray gene expression analysis in identifying gene ontologies associated with CFS.

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6793-5-5 contains supplementary material, which is available to authorized users.

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Autor: Toni Whistler - James F Jones - Elizabeth R Unger - Suzanne D Vernon

Fuente: https://link.springer.com/



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