Creation and disruption of protein features by alternative splicing - a novel mechanism to modulate functionReportar como inadecuado

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Genome Biology

, 6:R58

First Online: 22 June 2005Received: 25 February 2005Revised: 19 April 2005Accepted: 09 May 2005


BackgroundAlternative splicing often occurs in the coding sequence and alters protein structure and function. It is mainly carried out in two ways: by skipping exons that encode a certain protein feature and by introducing a frameshift that changes the downstream protein sequence. These mechanisms are widespread and well investigated.

ResultsHere, we propose an additional mechanism of alternative splicing to modulate protein function. This mechanism creates a protein feature by putting together two non-consecutive exons or destroys a feature by inserting an exon in its body. In contrast to other mechanisms, the individual parts of the feature are present in both splice variants but the feature is only functional in the splice form where both parts are merged. We provide evidence for this mechanism by performing a genome-wide search with four protein features: transmembrane helices, phosphorylation and glycosylation sites, and Pfam domains.

ConclusionWe describe a novel type of event that creates or removes a protein feature by alternative splicing. Current data suggest that these events are rare. Besides the four features investigated here, this mechanism is conceivable for many other protein features, especially for small linear protein motifs. It is important for the characterization of functional differences of two splice forms and should be considered in genome-wide annotation efforts. Furthermore, it offers a novel strategy for ab initio prediction of alternative splice events.

Electronic supplementary materialThe online version of this article doi:10.1186-gb-2005-6-7-r58 contains supplementary material, which is available to authorized users.

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Autor: Michael Hiller - Klaus Huse - Matthias Platzer - Rolf Backofen


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