Th1-Th2 cytokine pattern in bronchoalveolar lavage fluid and induced sputum in pulmonary sarcoidosisReportar como inadecuado

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BMC Pulmonary Medicine

, 5:8

First Online: 24 June 2005Received: 19 May 2005Accepted: 24 June 2005


BackgroundSarcoidosis is thought to be a T-helper type 1 cytokine Th2 cytokine mediated disorder. Induced sputum IS has been proposed as a useful non-invasive method, mainly for the assessment of the airway diseases. The aim of this study was to explore induced sputum IS CD4Th1 T-lymphocyte subpopulation and to compare them with those of bronchoalveolar lavage fluid BALF in patients with sarcoidosis.

MethodsWe studied prospectively 21 patients 12 female, 9 male of median age 46 yr range, 25–65 with sarcoidosis and 10 normal subjects 5 female, 5 male of median age 39 yr range, 26–60. IS was performed with hypertonic saline solution using an ultrasonic nebulizer. BALF was performed within 10 days of IS. After stimulation of sputum lymphocytes with phorbol-myristate-acetate, we used double immunocytochemical methods to identify CD4 IFN-γ positive and IL-4 positive cells Th1 and Th2, respectively.

ResultsSarcoidosis patients had an increased number of CD4+ -IFN-γ producing cells in IS p = 0.003 and BALF p = 0.01 in comparison with normal subjects. No significant differences were detected between CD4+ -IL-4 cells in BALF p = 0.053, NS and IS p = 0.46, NS between sarcoidosis patients and healthy controls. The ratio of Th1 to Th2 cells in BALF and IS was statistically different in sarcoidosis when compared with normal subjects p = 0.007 in BALF and IS. A significant correlation was found between CD4+ IFN-γ positive cells in IS and those in BALF in sarcoidosis patients r = 0.685, p = 0.0006.

ConclusionThese data suggests that a Th1-like cytokine pattern can be observed in CD4+ T-lymphocytes in IS in patients with pulmonary sarcoidosis. Further studies are needed to explore the value of IS vs BALF in the follow-up of these patients.

AbbreviationsBALFBronchoalveolar Lavage Fluid

FEV1 % predForced expiratory volume within the first second % predicted

FVC % predForced vital capacity % predicted

ILDInterstitial Lung Disease

KCOCarbon monoxide transfer coefficient

TNFTumor Necrosis Factor

IFN-?Interferon gamma

ISInduced Sputum

TBSTris-Buffered Saline

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2466-5-8 contains supplementary material, which is available to authorized users.

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Autor: Ioanna Tsiligianni - Katerina M Antoniou - Despina Kyriakou - Nikolaos Tzanakis - George Chrysofakis - Nikolaos M Siafakas


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