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BMC Molecular Biology

, 6:21

First Online: 17 November 2005Received: 22 July 2005Accepted: 17 November 2005


BackgroundSalmonid fishes are among the most widely studied model fish species but reports on systematic evaluation of reference genes in qRT-PCR studies is lacking.

ResultsThe stability of six potential reference genes was examined in eight tissues of Atlantic salmon Salmo salar, to determine the most suitable genes to be used in quantitative real-time RT-PCR analyses. The relative transcription levels of genes encoding 18S rRNA, S20 ribosomal protein, β-actin, glyceraldehyde-3P-dehydrogenase GAPDH, and two paralog genes encoding elongation factor 1A EF1AA and EF1AB were quantified in gills, liver, head kidney, spleen, thymus, brain, muscle, and posterior intestine in six untreated adult fish, in addition to a group of individuals that went through smoltification. Based on calculations performed with the geNorm VBA applet, which determines the most stable genes from a set of tested genes in a given cDNA sample, the ranking of the examined genes in adult Atlantic salmon was EF1AB>EF1AA>β-actin>18S rRNA>S20>GAPDH. When the same calculations were done on a total of 24 individuals from four stages in the smoltification process presmolt, smolt, smoltified seawater and desmoltified freshwater, the gene ranking was EF1AB>EF1AA>S20>β-actin>18S rRNA>GAPDH.

ConclusionOverall, this work suggests that the EF1AA and EF1AB genes can be useful as reference genes in qRT-PCR examination of gene expression in the Atlantic salmon.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2199-6-21 contains supplementary material, which is available to authorized users.

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Autor: Pål A Olsvik - Kai K Lie - Ann-Elise O Jordal - Tom O Nilsen - Ivar Hordvik

Fuente: https://link.springer.com/

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