DAX-1 expression in human breast cancer: comparison with estrogen receptors ER-α, ER-β and androgen receptor statusReportar como inadecuado

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Breast Cancer Research

, 6:R140

First Online: 13 February 2004Received: 05 November 2003Revised: 14 January 2004Accepted: 22 January 2004


BackgroundSo far there have been no reports on the expression pattern of DAX-1 d osage-sensitive sex reversal, a drenal hypoplasia critical region, on chromosome X , gene 1 in human breast cells and its relationship to the estrogen receptors, ER-α and ER-β, and the androgen receptor AR.

MethodsIn this study we evaluated, by immunohistochemistry and Western blot analysis, the presence and distribution of DAX-1 in benign breast disease BBD, in situ carcinoma CIS, and ductal and lobular breast carcinomas.

ResultsIn BBD and breast carcinomas, DAX-1 was present in both the nuclei and the cytoplasm of epithelial cells, although in infiltrative carcinomas the percentage of nuclear immunoreaction was higher than in CIS. An important relation was observed between DAX-1 and AR expression and between this orphan receptor and nodal status.

ConclusionDAX-1 might modify the AR and ER-β intracellular location, and because a direct positive relation between the expression of these three receptors was found it could be assumed that the presence of DAX-1 in neoplastic cells might indicate a possible failure of endocrine therapies.

Keywordsandrogen receptor breast cancer DAX-1 estrogen receptor-α estrogen receptor-β AbbreviationsARandrogen receptor

BBDbenign breast disease

CISin situ carcinoma

DAXdosage-sensitive sex reversal adrenal hypoplasia critical region, on chromosome X

ERestrogen receptor

NDSnormal donkey serum

TBSTris-buffered saline.

Electronic supplementary materialThe online version of this article doi:10.1186-bcr766 contains supplementary material, which is available to authorized users.

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Autor: Isabel Conde - Juan M Alfaro - Benito Fraile - Antonio Ruíz - Ricardo Paniagua - Maria I Arenas

Fuente: https://link.springer.com/

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