Cyclin B Translation Depends on mTOR Activity after Fertilization in Sea Urchin EmbryosReportar como inadecuado

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* Corresponding author 1 LBI2M - Laboratoire de Biologie Intégrative des Modèles Marins

Abstract : The cyclin B-CDK1 complex is a key regulator of mitotic entry. Using PP242, a specific ATP-competitive inhibitor of mTOR kinase, we provide evidence that the mTOR signalling pathway controls cyclin B mRNA translation following fertilization in Sphaerechinus granu-laris and Paracentrotus lividus. We show that PP242 inhibits the degradation of the cap-dependent translation repressor 4E-BP eukaryotic initiation factor 4E-Binding Protein. PP242 inhibits global protein synthesis, delays cyclin B accumulation, cyclin B-CDK1 complex activation and consequently entry into the mitotic phase of the cell cycle triggered by fertilization. PP242 inhibits cyclin B mRNA recruitment into active polysomes triggered by fertilization. An amount of cyclin B mRNA present in active polysomes appears to be insensitive to PP242 treatment. Taken together, our results suggest that, following sea urchin egg fertilization, cyclin B mRNA translation is controlled by two independent mechanisms: a PP242-sensitive and an additional PP242-insentitive mechanism.

Autor: Héloïse Chassé - Odile Mulner-Lorillon - Sandrine Boulben - Virginie Glippa - Julia Morales - Patrick Cormier -



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