The Timing of Timezyme Diversification in VertebratesReportar como inadecuado




The Timing of Timezyme Diversification in Vertebrates - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 BIOM - Biologie intégrative des organismes marins

Abstract : All biological functions in vertebrates are synchronized with daily and seasonal changes in the environment by the time keeping hormone melatonin. Its nocturnal surge is primarily due to the rhythmic activity of the arylalkylamine N-acetyl transferase AANAT, which thus became the focus of many investigations regarding its evolution and function. Various vertebrate isoforms have been reported from cartilaginous fish to mammals but their origin has not been clearly established. Using phylogeny and synteny, we took advantage of the increasing number of available genomes in order to test whether the various rounds of vertebrate whole genome duplications were responsible for the diversification of AANAT. We highlight a gene secondary loss of the AANAT2 in the Sarcopterygii, revealing for the first time that the AAANAT1-2 duplication occurred before the divergence between Actinopterygii bony fish and Sarcopterygii tetrapods, lobe-finned fish, and lungfish. We hypothesize the teleost-specific whole genome duplication WDG generated the appearance of the AANAT1a-1b and the AANAT2- 29paralogs, the 29 isoform being rapidly lost in the teleost common ancestor ray-finned fish. We also demonstrate the secondary loss of the AANAT1a in a Paracantopterygii Atlantic cod and of the 1b in some Ostariophysi zebrafish and cave fish. Salmonids present an even more diverse set of AANATs that may be due to their specific WGD followed by secondary losses. We propose that vertebrate AANAT diversity resulted from 3 rounds of WGD followed by previously uncharacterized secondary losses. Extant isoforms show subfunctionalized localizations, enzyme activities and affinities that have increased with time since their emergence.





Autor: Damien Cazaméa-Catalan - Laurence Besseau - Jack Falcón - Elodie Magnanou -

Fuente: https://hal.archives-ouvertes.fr/



DESCARGAR PDF




Documentos relacionados