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BMC Immunology

, 5:16

First Online: 03 August 2004Received: 13 February 2004Accepted: 03 August 2004

Abstract

BackgroundExtracellular nucleotides ATP, ADP, UTP and UDP exert a wide range of biological effects in blood cells mediated by multiple ionotropic P2X receptors and G protein-coupled P2Y receptors. Although pharmacological experiments have suggested the presence of several P2 receptor subtypes on monocytes and lymphocytes, some results are contradictory. Few physiological functions have been firmly established to a specific receptor subtype, partly because of a lack of truly selective agonists and antagonists. This stimulated us to investigate the expression of P2X and P2Y receptors in human lymphocytes and monocytes with a newly established quantitative mRNA assay for P2 receptors. In addition, we describe for the first time the expression of P2 receptors in CD34 stem and progenitor cells implicating a potential role of P2 receptors in hematopoietic lineage and progenitor-stem cell function.

ResultsUsing a quantitative mRNA assay, we assessed the hypothesis that there are specific P2 receptor profiles in inflammatory cells. The P2X4 receptor had the highest expression in lymphocytes and monocytes. Among the P2Y receptors, P2Y12 and P2Y2 had highest expression in lymphocytes, while the P2Y2 and P2Y13 had highest expression in monocytes. Several P2 receptors were expressed P2Y2, P2Y1, P2Y12, P2Y13, P2Y11, P2X1, P2X4 in CD34+ stem and progenitor cells.

ConclusionsThe most interesting findings were the high mRNA expression of P2Y12 receptors in lymphocytes potentially explaining the anti-inflammatory effects of clopidogrel, P2Y13 receptors in monocytes and a previously unrecognised expression of P2X4 in lymphocytes and monocytes. In addition, for the first time P2 receptor mRNA expression patterns was studied in CD34 stem and progenitor cells. Several P2 receptors were expressed P2Y2, P2Y1, P2Y12, P2Y13, P2Y11, P2X1, P2X4, indicating a role in differentiation and proliferation. Thus, it is possible that specific antibodies to P2 receptors could be used to identify progenitors for monocytes, lymphocytes and megakaryocytes.

KeywordsP2 receptor real-time PCR lymphocytes monocytes CD34 cells hematopoietic stem cells Electronic supplementary materialThe online version of this article doi:10.1186-1471-2172-5-16 contains supplementary material, which is available to authorized users.

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Autor: Lingwei Wang - Sten Eirik W Jacobsen - Anders Bengtsson - David Erlinge

Fuente: https://link.springer.com/



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