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Reproductive Biology and Endocrinology

, 2:73

First Online: 17 October 2004Received: 03 June 2004Accepted: 17 October 2004


BackgroundCarbonic anhydrase CA classically catalyses the reversible hydration of dissolved CO2 to form bicarbonate ions and protons. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems.

MethodsThe present study was designed to investigate the expression of transmembrane CAs, CA IX and XII, in the mouse uterus, ovary and placenta. The expression of CA IX and XII was examined by immunoperoxidase staining method and western blotting. CA II and XIII served as positive controls since they are known to be present in the mouse reproductive tract.

ResultsThe data of our study indicated that CA XII is expressed in the mouse endometrium. Only very faint signal was observed in the corpus luteum of the ovary and the placenta remained mainly negative. CA IX showed weak reaction in the endometrial epithelium, while it was completely absent in the ovary and placenta.

ConclusionThe conservation of CA XII expression in both mouse and human endometrium suggests a role for this isozyme in reproductive physiology.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-7827-2-73 contains supplementary material, which is available to authorized users.

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Autor: Piritta Hynninen - Jonna M Hämäläinen - Silvia Pastorekova - Jaromir Pastorek - Abdul Waheed - William S Sly - Eija To


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