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BMC Gastroenterology

, 4:33

First Online: 29 December 2004Received: 01 July 2004Accepted: 29 December 2004


BackgroundNidation of floating tumour cells initiates peritoneal carcinosis and limits prognosis of gastro-intestinal tumours. Adhesion of tumour cells to extracellular matrix components is a pivotal step in developing peritoneal dissemination of intraabdominal malignancies. Since phospholipids efficaciously prevented peritoneal adhesion formation in numerous animal studies we investigated their capacity to reduce adhesions of gastric cancer cells to extracellular matrix components ECM.

MethodsHuman gastric cancer cells NUGC-4, Japanese Cancer Research Resources Bank, Tokyo, Japan were used in this study. Microtiter plates were coated with collagen IV coll, laminin ln and fibronectin fn. Non-specific protein binding of the coated wells was blocked by adding 1% w-v BSA 4°C, 12 h and rinsing the wells with Hepes buffer. 50.000 tumour cells in 100 μl medium were seeded into each well. Beside the controls, phospholipids were added in concentrations of 0.05, 0.1, 0.5, 0.75 and 1.0-100 μl medium. After an incubation interval of 30 min, attached cells were fixed and stained with 0.1% w-v crystal violet. The dye was resuspended with 50 μl of 0.2% v-v Triton X-100 per well and colour yields were then measured by an ELISA reader at 590 nm. Optical density OD showed a linear relationship to the amount of cells and was corrected for dying of BSA-polystyrene without cells.

ResultsThe attachment of gastric cancer cells to collagen IV, laminin, and fibronectin could be significantly reduced up to 53% by phospholipid concentrations of 0.5 mg-100 μl and higher.

ConclusionThese results, within the scope of additional experimental studies on mice and rats which showed a significant reduction of peritoneal carcinosis, demonstrated the capacity of phospholipids in controlling abdominal nidation of tumour cells to ECM components. Lipid emulsions may be a beneficial adjunct in surgery of gastrointestinal malignancies.

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Autor: Marc Jansen - Karl-Heinz Treutner - Britta Schmitz - Jens Otto - Petra Lynen Jansen - S Neuss - Volker Schumpelick


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