Polymorphism in two merozoite surface proteins of Plasmodium falciparum isolates from GabonReportar como inadecuado




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Malaria Journal

, 2:12

First Online: 09 May 2003Received: 03 March 2003Accepted: 09 May 2003

Abstract

BackgroundPlasmodium falciparum antigenic diversity and polymorphism confuses the issue of antimalarial vaccine development. Merozoite surface protein MSP-1 and -2 are two highly polymorphic vaccine candidates. Characterisation of their precise polymorphism in endemic regions may facilitate the design of an effective vaccine.

MethodsIsolates obtained in 52 Gabonese children presenting with uncomplicated malaria were genotyped by nested-PCR of msp-1 block 2, and msp-2 block 3, to analyze both parasite population polymorphism and clone fluctuations.

ResultsTwenty-five and 19 different alleles were respectively obtained for msp-1 and msp-2 loci, the RO33 family of msp-1 being poorly polymorphic. Four cases of non-random distribution of alleles were reported of the FC27, and-or 3D7 families of msp-2. All but two isolates were composed of more than one genotype, and the multiplicity of infection MOI was 4.0. Neither parasite density nor age was related to MOI. Clone fluctuations were studied for ten subjects who were sampled again at reappearance of parasites in blood. Disappearance and reappearance of alleles were observed following treatment, suggesting difficulties in assessing polymorphism and in distinguishing reinfection from recrudescence.

ConclusionP. falciparum polymorphism is extensive in Southeast Gabon, and most of infections are composed of multiple clones. The fluctuation of clones contributes to parasite diversity.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2875-2-12 contains supplementary material, which is available to authorized users.

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Autor: Agnès Aubouy - Florence Migot-Nabias - Philippe Deloron

Fuente: https://link.springer.com/







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