Establishment of a monoclonal antibody for human LXRα: Detection of LXRα protein expression in human macrophagesReportar como inadecuado




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Nuclear Receptor

, 1:1

First Online: 09 May 2003Received: 01 April 2003Accepted: 09 May 2003

Abstract

Liver X activated receptor alpha LXRα forms a functional dimeric nuclear receptor with RXR that regulates the metabolism of several important lipids, including cholesterol and bile acids. As compared with RXR, the LXRα protein level in the cell is low and the LXRα protein itself is very hard to detect. We have previously reported that the mRNA for LXRα is highly expressed in human cultured macrophages. In order to confirm the presence of the LXRα protein in the human macrophage, we have established a monoclonal antibody against LXRα, K-8607. The binding of mAb K-8607 to the human LXRα protein was confirmed by a wide variety of different techniques, including immunoblotting, immunohistochemistry, and electrophoretic mobility shift assay EMSA. By immunoblotting with this antibody, the presence of native LXR protein in primary cultured human macrophage was demonstrated, as was its absence in human monocytes. This monoclonal anti-LXRα antibody should prove to be a useful tool in the analysis of the human LXRα protein.

Electronic supplementary materialThe online version of this article doi:10.1186-1478-1336-1-1 contains supplementary material, which is available to authorized users.

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Autor: Yuichiro Watanabe - Toshiya Tanaka - Yasutoshi Uchiyama - Tetsu Takeno - Akashi Izumi - Hisahiko Yamashita - Junko Kumakura

Fuente: https://link.springer.com/







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