Interleukin-1 IL-1 system gene expression in granulosa cells: kinetics during terminal preovulatory follicle maturation in the mareReportar como inadecuado




Interleukin-1 IL-1 system gene expression in granulosa cells: kinetics during terminal preovulatory follicle maturation in the mare - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Reproductive Biology and Endocrinology

, 1:42

First Online: 16 May 2003Received: 09 April 2003Accepted: 16 May 2003

Abstract

BackgroundA growing body of evidences suggests that the ovary is a site of inflammatory reactions, and thus, ovarian cells could represent sources and targets of the interleukin-1 IL-1 system. The purpose of this study was to examine the IL-1 system gene expressions in equine granulosa cells, and to study the IL-1β content in follicular fluid during the follicle maturation. For this purpose, granulosa cells and follicular fluids were collected from the largest follicle at the early dominance stage diameter 24 ± 3 mm or during the preovulatory maturation phase, at T0 h, T6 h, T12 h, T24 h and T34 h after induction of ovulation. Cells were analysed by RT-PCR and follicular fluids were studied by gel electrophoresis and immunoblotting.

ResultsWe demonstrated that interleukin-1β IL-1β, interleukin-1 receptor 2 IL-1R2 and interleukin-1 receptor antagonist IL-1RA genes are expressed in equine granulosa cells. We observed that the IL-1β and IL-1RA mRNA content changed in granulosa cells during the terminal follicular maturation whereas IL-1R2 mRNA did not vary. In follicular fluid, IL-1β content fluctuated few hours after induction of ovulation.

ConclusionsThe expression of IL-1β gene in granulosa cells and the follicular fluid IL-1β content seem to be regulated by gonadotropins suggesting that IL-1β could be an intermediate paracrine factor involved in ovulation.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-7827-1-42 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Alain Martoriati - Nadine Gérard

Fuente: https://link.springer.com/







Documentos relacionados